Rai™ Taq DNA Polymerase
Overview
Rai™ Taq DNA Polymerase is a high-performance recombinant enzyme designed to enhance the amplification of DNA in PCR applications. Rai™ Taq DNA polymerase combines the robust properties of Thermus aquaticus Taq DNA polymerase with the processivity-enhancing DNA binding domain. The fusion of Taq DNA polymerase and domain results in improved speed, fidelity, and reliability.
Product Source
An E. coli strain carrying a recombinant fusion of Thermus aquaticus YT-1 Taq DNA polymerase gene and DNA binding domain gene.
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Reaction Conditions
See Protocols section for detailed information.
Application Features
• Routine PCR applications
• Colony PCR
• Primer Extension
• Improved performance (compared to Taq DNA polymerase) under challenging PCR conditions, including high temperatures, complex templates, and high inhibitor concentrations.
• More accurate and longer templates
• Taq DNA Polymerase shows deoxynucleotidyl transferase activity, often leading to the addition of extra adenines at the 3′-end of PCR products.
Concentration: 5.000 U/ml
Usage Concentration: 1.25 U/ 50 µl reaction
Reaction Buffer: 1 X UTaq Buffer
Sensitive to Heat Inactivation: No
Optimal Extension Temperature: 68 °C
5’-3’ Nuclease Activity: Yes
| Cat. No. | Component | Quantity | Storage | Concentration |
|---|---|---|---|---|
| MCE0201-S
(500 unit) |
Rai Taq DNA Polymerase | 1 x 0.10 ml | -20˚C | 5,000 U/ml |
| UTaq Buffer | 1 x 1.5 ml | -20˚C | 10X | |
| 25 mM MgCl2 | 1 x 1.5 ml | 25˚C | – | |
| MCE0201-L
(2000 unit) |
Rai Taq DNA Polymerase | 1 x 0.40 ml | -20˚C | 5,000 U/ml |
| UTaq Buffer | 2 x 1.5 ml | -20˚C | 10X | |
| 25 mM MgCl2 | 2 x 1.5 ml | 25˚C | – |
| Reagent | Amount |
|---|---|
| Rai™ DNA polymerase (5.000 U/ml) | 0.25 µl |
| UTaq Buffer | 5 µl |
| dNTP Mix (10 mM) | 1 µl |
| Forward Primer (10 µM) | 2.5 µl* |
| Reverse Primer (10 µM) | 2.5 µl* |
| 25 mM MgCl2 (optional) | ** |
| DNA template | 1pg-1µg*** |
| Water (Ultra Pure) | X**** |
| Total Reaction Volume | 50 µl |
***Ultra-pure water is added to bring the total reaction volume to 50 µl.
Note: Enzymes are supplied with 25 mM MgCl2 Solution and UTaq Buffer (10X) suitable for single and double digestions.
Research Use Only
Related Additional Materials
| Product | Cat. No. | Quantity | Storage | Concentration |
|---|---|---|---|---|
| UTaq Buffer | MCR0003 | 1 x 1.5 ml | -20 ˚C | 10X |
| 25 mM MgCl2 | MCR0004 | 1 x 1.5 ml | 25 ˚C | – |
| dNTP Mix (10mM) | MCR0005 | 4 x 0.2 ml | -20 ˚C | – |